6.4 – How Lipids Work

Learning Objectives

  • Explain how the structure of each lipid impacts its function as it relates to health and disease.
  • Compare and contrast the structure of the following types of fats: triglycerides (monounsaturated, polyunsaturated, omega-3, and omega-6), phospholipids, and sterols.
  • Explain how the fatty acids you consume relate to the fatty-acid composition of your body fat.


Lipids are unique organic compounds, each serving key roles and performing specific functions within the body. As we discuss the various types of lipids (triglycerides, phospholipids, and sterols) in further detail, we will compare their structures and functions and examine their impact on human health.


Triglyceride Structure and Functions

Triglycerides are the main form of lipid found in the body and in the diet. Fatty acids and glycerol are the building blocks of triglycerides. Glycerol is a thick, smooth, syrupy compound that is often used in the food industry. To form a triglyceride, a glycerol molecule is joined by three fatty acid chains. Triglycerides contain varying mixtures of fatty acids.

Chemical structure of glycerol, 3 fatty acids and triglyceride (triester of glycerol)
Figure 6.4.1 Triglyceride components.

Fatty Acids

Fatty acids determine if the compound is solid or liquid at room temperature. Fatty acids consist of a carboxylic acid (−OOH) group on one end of a carbon chain and a methyl group (−CH3) on the other end. Fatty acids can differ from one another in two important ways—carbon chain length and degree of saturation.


It’s All in the Chain

Fatty acids have different chain lengths and different compositions. Foods have fatty acids with chain lengths between four and twenty-four carbons and most of them contain an even number of carbon atoms. When the carbon chain length is shorter, the melting point of the fatty acid becomes lower—and the fatty acid becomes more liquid.

Chemical structures of a Saturated, Monounsaturated, and Polyunsaturated Fat
Figure 6.4.2 Structures of a Saturated, Monounsaturated, and Polyunsaturated Fat

A methyl group is on the left. A carboxyl group is on the right. The carbon atoms of those are connected to 2 carbon atoms between the methyl and carboxyl groups, so there is a chain of carbon atoms from left to right.

Degrees of Saturation

Fatty acid chains are held together by carbon atoms that attach to each other and to hydrogen atoms. The term saturation refers to whether or not a fatty acid chain is filled (or “saturated”) to capacity with hydrogen atoms. If each available carbon bond holds a hydrogen atom we call this a saturated fatty acid chain. All carbon atoms in such a fatty acid chain are bonded with single bonds. Sometimes the chain has a place where hydrogen atoms are missing. This is referred to as the point of unsaturation.

When one or more bonds between carbon atoms are a double bond (C=C), that fatty acid is called an unsaturated fatty acid, as it has one or more points of unsaturation. Any fatty acid that has only one double bond is a monounsaturated fatty acid, an example of which is olive oil (75 percent of its fat is monounsaturated). Monounsaturated fats help regulate blood cholesterol levels, thereby reducing the risk of heart disease and stroke. A polyunsaturated fatty acid is a fatty acid with two or more double bonds or two or more points of unsaturation. Soybean oil contains high amounts of polyunsaturated fatty acids. Both monounsaturated fats and polyunsaturated fats provide nutrition that is essential for normal cell development and healthy skin.

Chemical structure of stearic acid, oleic acid and linoleic acid.
Figure 6.4.3 Chemical structure of stearic acid, oleic acid, and linoleic acid.

3 chemical structures are shown. 1. Stearic acid or C subscript 18 H subscript 36 O subscript 2: a saturated fat. 2. Oleic acid or C subscript 18 H subscript 34 O subscript 2: a monounsaturated fat with a double bond. 3. Linoleic acid or C subscript 18 H subscript 32 O subscript 2: a polyunsaturated fat with 2 double bonds. Foods that have a high percentage of saturated fatty acids tend to be solid at room temperature. Examples of these are the fats found in chocolate (stearic acid, an eighteen-carbon saturated fatty acid is a primary component) and meat. Foods rich in unsaturated fatty acids, such as olive oil (oleic acid, an eighteen-carbon unsaturated fatty acid, is a major component) tend to be liquid at room temperature. Flaxseed oil is rich in alpha-linolenic acid, which is an unsaturated fatty acid and becomes a thin liquid at room temperature.

Knowing the connection between chain length, degree of saturation and the state of the fatty acid (solid or liquid) is important for making food choices. If you decide to redirect your intake of fat products, choosing unsaturated fat is more beneficial than choosing a saturated fat. Examples of foods high or rich in unsaturated fats include avocados, olive and canola oils(monounsaturated), most vegetable and fish oils (polyunsaturated), nuts and seeds.  Conversely, tropical oils are an exception to this rule in that they are high in saturated fat. Palm oil and coconut oil are both highly saturated and have been shown to raise blood cholesterol. Shortening, margarine, and commercially prepared products (in general) report to use only vegetable-derived fats in their processing. But even so, some of these contain fats that may be in the saturated and trans fat categories.


Cis or Trans Fatty Acids?

The introduction of a carbon double bond in a carbon chain, as in an unsaturated fatty acid, can result in different structures for the same fatty acid composition. When the hydrogen atoms are bonded to the same side of the carbon chain, it is called a cis fatty acid. Because the hydrogen atoms are on the same side, the carbon chain has a bent structure. Naturally occurring fatty acids usually have a cis configuration.

In a trans fatty acid, the hydrogen atoms are attached on opposite sides of the carbon chain. Unlike cis fatty acids, most trans fatty acids are not found naturally in foods but are a result of a process called hydrogenation. Hydrogenation is the process of adding hydrogen to the carbon double bonds, thus making the fatty acid saturated (or less unsaturated, in the case of partial hydrogenation). This is how vegetable oils are converted into semisolid fats for use in the manufacturing process.

According to the ongoing Harvard Nurses Health Study, trans fatty acids have been associated with increased risk for coronary heart disease because of the way they negatively impact blood cholesterol levels.1

Chemical structure of a bent cis fatty acid and a straighter trans fatty acid
Figure 6.4.4 Chemical structure of a bent cis fatty acid and a straighter trans-fatty acid

Interestingly, some naturally occurring trans fats do not pose the same health risks as their artificially engineered counterparts. These trans fats are found in ruminant animals such as cows, sheep, and goats, resulting in trans fatty acids being present in our meat, milk, and other dairy product supply.  It seems that any positive effect naturally occurring trans fats have are counteracted by the presence of the saturated fatty acid molecules in these animal products.1 Harvard School of Public Health. “It’s Time to End the Low-Fat Myth.” Introduction to “Fats and Cholesterol: Out with the Bad, In with the Good” in The Nutrition Source. Accessed June 21, 2019.


Nonessential and Essential Fatty Acids

Fatty acids are vital for the normal operation of all body systems. The circulatory system, respiratory system, integumentary system, immune system, brain, and other organs require fatty acids for proper function. The body is capable of synthesizing most of the fatty acids it needs from food. These fatty acids are known as nonessential fatty acids. However, there are some fatty acids that the body cannot synthesize and these are called essential fatty acids. It is important to note that the term nonessential fatty acids doesn’t mean unimportant; the classification is based solely on the ability of the body to synthesize the fatty acid.

Essential fatty acids must be obtained from food. They fall into two categories—omega-3 and omega-6. The 3 and 6 refer to the position of the first carbon double bond and the omega refers to the methyl end of the chain. Omega-3 and omega-6 fatty acids are precursors to important compounds called eicosanoids. Eicosanoids are powerful hormone-like molecules that control many other hormones and important body functions, such as in the central nervous system and the immune system. Eicosanoids derived from omega-6 fatty acids are known to increase blood pressure, immune response, and inflammation. In contrast, eicosanoids derived from omega-3 fatty acids are known to have heart-healthy effects. Given the contrasting effects of the omega-3 and omega-6 fatty acids, a proper dietary balance between the two must be achieved to ensure optimal health benefits.

Essential fatty acids play an important role in the life and death of cardiac cells, immune system function, and blood pressure regulation. Some excellent sources of omega-3 and omega-6 essential fatty acids are fish, flaxseed oil, hemp, walnuts, and leafy vegetables. Because these essential fatty acids are easily accessible, an essential fatty acid deficiency is extremely rare.


Tools For Change

It is important to strike a proper balance between omega-3 and omega-6 fats in your diet. Research suggests that a diet that is too high in omega-6 fats distorts the balance of proinflammatory agents, promoting chronic inflammation and causing the potential for health problems such as asthma, arthritis, allergies, or diabetes. Omega-6 fats compete with omega-3 fats for enzymes and will actually replace omega-3 fats. The typical western diet is characterized by excessive consumption of foods high in omega-6 fatty acids. To gain proper balance between the two, increase your omega-3 fat intake by eating more fatty fish or other sources of omega-3 fatty acids at least two times per week.

Types of food shown: mackerel, salmon, spinach, walnuts, shrimp, oysters, soybeans.
Figure 6.4.5 There are many sources of omega-3 foods.


Fatty-Acid Composition in the Diet and in the Body

The fatty-acid profile of the diet directly correlates to the tissue lipid profile of the body. Instead of focusing on the quantity of dietary fat eaten, it is more important to focus on the type of dietary fat ingested as well as the entire dietary pattern of eating. Substituting unsaturated fats for saturated fats is the best way to reduce your risk of cardiovascular disease.

1 Liu AG, Ford NA, Hu FB, Zelman KM, Mozaffarian D, Kris-Etherton PM. A healthy approach to dietary fats: understanding the science and taking action to reduce consumer confusion. Nutr J. 2017;16(1):53. Published 2017 Aug 30. DOI:10.1186/s12937-017-0271-4. Accessed June 21, 2019.




Like triglycerides, phospholipids have a glycerol backbone. But unlike triglycerides, phospholipids are diglycerides (two fatty-acid molecules attached to the glycerol backbone) while their third fatty-acid chain has a phosphate group coupled with a nitrogen-containing group. This unique structure makes phospholipids water-soluble. Phospholipids are what we call amphiphilic—the fatty-acid sides are hydrophobic (dislike water) and the phosphate group is hydrophilic (likes water).

In the body, phospholipids bind together to form cell membranes. The nature of phospholipids governs their function as components of cell membranes. The phospholipids form a double layer in cell membranes, thus effectively protecting the inside of the cell from the outside environment while at the same time allowing for transport of fat and water through the membrane.

Phospholipids are ideal emulsifiers that can keep oil and water mixed. Emulsions are mixtures of two liquids that do not mix. Without emulsifiers, the fat and water content would be somewhat separate within the food. Lecithin (phosphatidylcholine), found in egg yolk, honey, and mustard, is a popular food emulsifier. Mayonnaise demonstrates lecithin’s ability to blend vinegar and oil to create the stable, spreadable condiment that so many enjoy. Food emulsifiers play an important role in making the appearance of food appetizing. Adding emulsifiers to sauces and creams not only enhances their appearance but also increases their freshness.

Lecithin’s crucial role within the body is clear because it is present in every cell throughout the body; 28 percent of brain matter is composed of lecithin and 66 percent of the fat in the liver is lecithin. Many people attribute health-promoting properties to lecithins, such as its ability to lower blood cholesterol and aid with weight loss. There are several lecithin supplements on the market broadcasting these claims. However, as the body can make most phospholipids, it is not necessary to consume them in a pill. The body makes all of the lecithins that it needs.


Video 6.4.1 – “The Food Lab: How to Make Mayonnaise in Two Minutes or Less”

Author: J. Kenji López-Alt
Published on Aug 12, 2016

For accessibility purposes, a set of captions has been made for this video, which otherwise had captions automatically generated. Here you can watch the video using these captions.


Sterols have a very different structure from triglycerides and phospholipids. Most sterols do not contain any fatty acids but rather multi-ring structures. They are complex molecules that contain interlinking rings of carbon atoms, with side chains of carbon, hydrogen, and oxygen attached. Cholesterol is the best-known sterol because of its role in heart disease. It forms a large part of the plaque that narrows the arteries in atherosclerosis. In stark contrast, cholesterol does have specific beneficial functions to perform in the body. Like phospholipids, cholesterol is present in all body cells as it is an important substance in cell membrane structure. Approximately 25 percent of the cholesterol in the body is localized in brain tissue. Cholesterol is used in the body to make a number of important things, including vitamin D, glucocorticoids, and sex hormones, progesterone, testosterone, and estrogens. Notably, the sterols found in plants resemble cholesterol in structure. However, plant sterols inhibit cholesterol absorption in the human body, which can contribute to lower cholesterol levels.

Although cholesterol is preceded by its infamous reputation, it is clearly a vital substance in the body that poses a concern only when there is an excess accumulation of it in the blood. The body synthesizes the majority of the cholesterol present in our body


Key Takeaways

    • Three fatty acids combine with one glycerol to make a triglyceride, the body’s storage form of fat.
    • The carbon chain in fatty acids can be saturated (filled with hydrogen), monounsaturated (with one point of unsaturation and possessing one carbon-carbon double bond), or polyunsaturated (with more than one point of unsaturation and possessing two or more double-carbon bonds)
    • The structure and length of a fatty acid determine whether it is solid or liquid at room temperature.
    • Essential fatty acids cannot be synthesized by the body and must be included in the diet. Omega-3 and omega-6 fatty acids have opposing functions and must be consumed in proper balance to promote health.
    • Fatty acids in food influence the composition of fatty acids in the body.
    • Phospholipids are diglycerides with a phosphate-nitrogen group in place of the third fatty-acid chain. They are ideal emulsifiers as they keep oil and water mixed.
    • Sterols contain a multi-ring structure. Cholesterol serves important body functions such as vitamin D and hormone synthesis and is important in the cell membrane and brain structure.


Discussion Starters

    • List the oils, snacks, spreads, or sauces in your home. Do they contain saturated or unsaturated fatty acids?
    • Name the characteristic that differentiates a triglyceride from a phospholipid. Describe the properties of phospholipids. Discuss their role in forming cell membranes. Explain their role as an emulsifier.
    • Think about your favorite restaurant and the food you like to order there. What type of fatty acids does a typical meal that you eat there have? Discuss the health benefits and possible drawbacks. What foods would you add to enhance your meal? What foods should you consider avoiding?
    • Consider ways you could substitute saturated fats for unsaturated fats in your diet. Give specific examples.


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Nutrition 100 Nutritional Applications for a Healthy Lifestyle Copyright © by Lynn Klees and Alison Borkowska is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, except where otherwise noted.

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